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Posted By: srinivas Member Level: Gold Posted Date: 29 May 2008
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2007 Andhra Pradesh State Jawaharlal Nehru Technological University Biotechnology II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 BIO PROCESS ENGINEERING-I Question paper
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Code No: RR222302 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
1. Mention different types of enzymes extracted from plant and their application.
[8+8]
2. (a) What is meant by immobilisation of cells? What are the advantages of immo-
bilised cell culture over suspension cell culture? [2+4]
(b) What is active immobilisation? Explain various matrices used for active
immobilisation. [2+4]
(c) Describe the methods employed for active cell immobilisation. [4]
3. Describe the use of precursors and metabolic regulators in media preparation.[16]
4. (a) Draw the schematic diagram of a typical continuous injector- flash cooler ster-
iliser.
(b) Draw the flow diagram of continuous strilisation system employing spiral heat
exchangers. [8+8]
5. Estimate the theoretical growth and product yield coefficients for ethanol fermen-
tation by S cerevisiae as described by the following reaction
C6H12O6
- - - - - - -- ! 2C2H5OH + 2CO2 [16]
6. Explain the following:
(a) Control sites in metabolism [8]
(b) Transfer of bioenergy via ATP. [8]
7. Explain Monod model application in the bioprocess engineering along with
applications. [16]
8. (a) Differentiate between competitive product inhibition and non-competitive prod-
uct inhibition.
(b) Explain the product inhibition on the product formation. [8+8]
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