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Posted By: sasi kala Member Level: Diamond Posted Date: 31 May 2008
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2007 Anna University B.Tech Industrial BioTechnology IB 241 — MOLECULAR BIOLOGY Question paper
B.E./B.Tech. DEGREE EXAMINATION,
Fourth Semester
Industrial Biotechnology
IB 241 — MOLECULAR BIOLOGY
Time : Three hours Maximum : 100 marks
Answer ALL questions.
PART A — (10 ? 2 = 20 marks)
1. Enumerate the differences between B-DNA and Z-DNA.
2. How is an initiator tRNA different from tRNAmet in E. coli?
3. Which two amino acids have a single codon? Give their codon sequence.
4. How is specificity of binding of various transcription factors to specific DNA sequences achieved?
5. What is the role of cAMP in the regulation of lac operon?
6. Why is ATP hydrolysis required for the unwinding of DNA by the dna B gene product of E. coli?
7. How is the kinetic complexity of eukaryotic DNA related to its chemical complexity?
8. A nuclear localization signal is inserted in the middle of the sequence of an
ER protein. Where will this ‘hybrid’ protein be localized in the cell?
9. Write briefly on retroviruses.
10. I have generated a strain of S. cerevisiae lacking a functional guanylyl transferase enzyme that caps the ends of mRNAs. Would this strain be viable? Explain.
PART B — (5 ? 16 = 80 marks)
11. Explain the mechanism of splicing of eukaryotic nuclear genes.
12. (a) Detail the steps involved in the targeting, translocation and localization of an endoplasmic reticulum lumen protein.
Or
(b) Detail the steps involved in initiation and elongation phases of translation in prokaryotes.
13. (a) Outline the mechanism of replication of the ends of linear
eukaryotic DNA.
Or
(b) Explain how the origin is fired and replication is regulated in E. coli.
14. (a) Explain in detail the regulation of the tryptophan operon in E. coli.
Or
(b) Detail the mechanism of genomic imprinting in mammals.
15. (a) Write notes on :
(i) Mutagenesis by deoxybromouridine.
(ii) Nucleotide excision repair.
Or
(b) Write notes on :
(i) Regulation of CI gene expression in lambda phage.
(ii) Zinc-finger proteins.
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