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Posted By: kishore Member Level: Gold Posted Date: 06 Jun 2008
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2007 Jawaharlal Nehru Technological University B.Tech Biotechnology PROTEIN ENGINEERING setno3 Question paper
1. What is Secondary Structure of protein? Discuss in details. [16]
2. What are the factors that affect the protein stability? [16]
3. (a) What are the protein which assist in protein folding.
(b) Write their classification and their function. [8+8]
4. Describe the action of restriction enzyme in detail. [16]
5. (a) What are proteases.
(b) Describe different classes of proteases with examples.
(c) Explain the action of serine proteases in details. [5+6+5]
6. Define antigenic determinants? Explain major categories of Antigenic determinant
on Immunoglobulins? [16]
7. Describe the advantages and disadvantages of oligonucleotide - directed mutagenesis
using PCR. [16]
8. Describe the basic principles with respect to protein design. [16]
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