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Posted By: kishore Member Level: Gold Posted Date: 06 Jun 2008
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2007 Jawaharlal Nehru Technological University B.Tech Biotechnology CHROMATOGRAPHIC SEPARATIONS setno1 Question paper
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1. Explain the terms:
(a) Retention chromatography
(b) Number of theoretical plates
(c) Retention parameters,
What is their significance? [16]
2. Give a schematic diagram of the chromatographic set-up and describe the functions
of the components. [16]
3. Give an account of the different operating modes of HPLC and its applications.
[16]
4. Define the terms:
(a) Total capacity
(b) Available capacity
(c) Dynamic capacity.
with respect to ion exchangers. How are they determined?
[16]
5. (a) Discuss the principle and applications involved in the practice of TLC.
(b) Write short notes on HPTLC principle and applications. [16]
6. Write short notes on types, principle and applications of different chromatographic
techniques. [16]
7. (a) Distinguish between qualitative and quantitative aspects of gas chromatogra-
phy.
(b) Give a schematic diagram of the gas chromatography and its applications.[16]
8. Describe the experimental setup and procedure of capillary electrophoresis. [16]
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