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Posted By: Sanjana Member Level: Gold Posted Date: 14 Jun 2008
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2007 Molecular biology may07 Question paper
Molecular Biology Module May 2007
SCHOOL OF BIOLOGY
UNIVERSITY OF ST ANDREWS
MOLECULAR BIOLOGY
MODULE BL1201
DEGREE EXAMINATION
May 2007
Time available: 2 hours
PLEASE WRITE YOUR MATRICULATION NUMBER ON EVERY SHEET, INCLUDING THE QUESTIONS YOU DO NOT ANSWER
SURNAME: ………………………………………………………………
GIVEN NAMES (in full): ………………………………………………...
MATRICULATION NUMBER: ………………………………………….
This examination paper comprises 10 questions, of which you
must answer 8.
All questions carry equal value.
Molecular Biology Module May 2007 Page 2
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
1. What does the phenomenon of denaturation tell us about protein conformation and the way in which it is stabilised?
If you continue overleaf please tick this box
Molecular Biology Module May 2007 Page 3
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
2. Describe how changes in pH can alter the affinity of haemoglobin for oxygen and explain why this is of physiological relevance.
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Molecular Biology Module May 2007 Page 4
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
3. Briefly outline the steps involved in transforming E. coli with a plasmid carrying an anti-biotic resistance gene. Describe what is happening at each step from the start of the experiment through to picking a colony.
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Molecular Biology Module May 2007 Page 5
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
4. a. Describe the roles of the following SIX proteins in DNA replication:
Topoisomerase
DNA polymerase
DNA ligase
Single strand binding proteins
RNA primase
DHA helicase (6 marks total)
b. Describe how the lagging strand is made during the process of DNA replication.
(4 marks total)
If you continue overleaf please tick this box
Molecular Biology Module May 2007 Page 6
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
5. With the aid of diagrams, describe the post-transcriptional modifications required to produce mRNA.
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Molecular Biology Module May 2007 Page 7
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
6. Describe briefly the functions of THREE of the following:
a. core histones.
b. gene promoters for RNA polymerase II.
c. translation elongation factors.
d. transfer RNA.
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Molecular Biology Module May 2007 Page 8
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
7. Briefly outline the steps involved in making a transgenic plant.
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Molecular Biology Module May 2007 Page 9
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
8. Describe how antigens are presented to T lymphocytes.
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Molecular Biology Module May 2007 Page 10
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
9. Write short notes on TWO of the following:
a. Lymph nodes.
b. Bacterial cell walls.
c. Clonal selection of B lymphocytes by antigens.
d. The IgM class of antibody.
e. The generalised life cycle of viruses.
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Molecular Biology Module May 2007 Page 11
PRINT YOUR MATRICULATION NUMBER HERE:
……………………………………………………………………………..
10. Actin monomers associate to form long filaments consisting of up to 5000 subunit pairs. These filaments are strong with respect to breaking but are readily assembled and disassembled at the ends. Based on a simple model for an actin filament, explain the thermodynamic basis for this phenomenon.
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