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Posted By: pradha s Member Level: Gold Posted Date: 27 Jun 2008
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2007 M.Phil(biotech) Question paper
DISTANCE EDUCATION
M.Phil. DEGREE EXAMINATION, DECEMBER 2007.
Biotechnology
RESEARCH METHODOLOGY AND LABORATORY TECHNIQUES
(2006 Batch)
Time : Three hours Maximum : 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
1. (a) Explain in detail principle of microscopic technique used for detection of live unstained cells. (10)
(b) Narrate the principle of flow-cytometry technique and its role in lymphocyte subset enumeration. (10)
2. Give an account on
(a) Molecular exclusion chromatography. (10)
(b) Affinity chromatography. (10)
3. (a) Explain in detail the immunoblotting technique used for diagnosis of HIV infection. (10)
(b) Give an account on types of ELISA and its application. (10)
4. (a) Describe in detail about measurement of radioactivity by liquid scintillation spectrophotometer. (10)
(b) Discuss the biological application of radioactive isotopes. (10)
5. Explain the principle of X-ray diffraction spectroscopy and its role in molecular structure analysis.
6. What is Proteomics? Give an account on different tools that aid proteomics.
7. Write the principle of automated DNA sequencing and its application in microbial diversity studies.
8. To verify whether a course in biotechnology improved performance, a similar test was given to 12 participants both before and after the course. The original marks recorded in alphabetical order of the participants were 44, 40, 61, 52, 32, 44, 70, 41, 67, 72, 53, and 72. After the course , the marks were in the same order, 53, 38, 69, 57, 46, 39,73, 48, 73, 74, 60, and 78. Using t-test find whether the course is useful.
—————
DISTANCE EDUCATION
M.Phil. DEGREE EXAMINATION, DECEMBER 2007.
Biotechnology
MICROBIAL, PLANT AND ANIMAL BIOTECHNOLOGY
(2006 Batch)
Time : Three hours Maximum : 100 marks
Answer any FIVE questions.
1. (a) What is strain selection. (5)
(b) Discuss the various screening methods for the selection of industrially important microbes. (15)
2. Give a detailed account of single cell protein and biogas production using microbes. (20)
3. (a) Discuss the principle of Agrobacterium mediated gene transfer using suitable diagram. (10)
(b) Explain the process of particle bombardment technology. (10)
4. (a) Define genetically modified animals with suitable examples. (10)
(b) Discuss the miscellaneous applications of transgenic animals. (10)
5. (a) How YAC and BAC is applied in human genome mapping. (10)
(b) Write in detail the applications of radiation hybrid
mapping and in situ hybridization in human genome mapping.
(10)
6. (a) Briefly discuss the role of microbes in the production of energy. (10)
(b) Write short note on single cell protein and its application in microbial biotechnology. (10)
7. (a) Brief the importance of selectable markers and reporter genes in constructing plant gene expression vectors.
(10)
(b) Discuss the types of gene silencing. (10)
8. (a) How Bacillus thuringiensis is used to generate insect pest resistant plants. (10)
(b) Write short note on expression of peptide hormones in transgenic plants. (10)
————
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