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Posted By: pradeep       Member Level: Diamond       Posted Date: 30 Aug 2008

2005 Jawaharlal Nehru Technological University III B.Tech. I Semester Regular Examinations, November -2005,ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY Question paper



Course: B.Tech Biotechnology   University: Jawaharlal Nehru Technological University




Code No: RR322302 Set No. 4
III B.Tech II Semester Supplementary Examinations,
November/December 2005
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. What is ”Phase shift” in Phase Contrast Microscopes? [16]
2. What do you mean by specimen preparation of TEM? [16]
3. How is blood sugar measured? [16]
4. Write notes on
(a) Stacking and Separation gels
(b) Sample loading in Electrophoresis [8+8]
5. Explain Northern Blotting. [16]
6. Explain the advantages and disadvantages of doing PCR based analysis. [16]
7. Differentiate between single and double Electroimmunodiffusion. [16]
8. What does COD mean? How is it determined?





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