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Posted Date: 30 Oct 2009 Posted By: Sree.... Member Level: Platinum
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2007 Andhra Pradesh State Jawaharlal Nehru Technological University Code No: R05312304 III B.Tech I Semester Regular Examinations, November 2007 GENETIC ENGINEERING [Set No. 2] Question paper
Code No: R05312304 Set No. 2
III B.Tech I Semester Regular Examinations, November 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
1. In which operon do you find the leader sequence? How does the translation of
leader sequence help in transcription control in certain operons? [16]
2. Explain the following terms:
(a) Regulatory sequences
(b) Enhancers
(c) reporter genes
(d) gene silencing. [4 × 4]
3. Comment on:
(a) LTR retroelements
(b) non LTR retroelements. [8 × 2]
4. How does one isolate the genomic DNA? What are the differences in DNA isolation
procedures in bacteria, plant cells and animal cells? [16]
5. Explain the recent developments in DNA sequencing. [16]
6. What are the different factors that one should take into consideration for obtaining
a successful amplification in PCR? [16]
7. What is the alternative to DNA arrays? Compare the technology and sensitivity
of DNA arrays and oligo chips. [16]
8. What are the limitations and advantages of gene therapy? Discuss in detail. [16]
1 of 1
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