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Posted By: Nitin Bajaj - Editor Member Level: Diamond Posted Date: 28 Mar 2008
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2007 Jawaharlal Nehru Technological University B.Tech BIO PROCESS ENGINEERING (Bio-Technology) - Set 3 Question paper
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II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 BIO PROCESS ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ?????
1. Discuss in detail about Chronological development of the fermentation industry. [4+12]
2. (a) What is aseptic operation and containment? (b) Describe a typical aseptic, aerobic fermentation process. (c) What is sparger? Describe di?erent spargers used in fermentors. [4] [4] [2+6]
3. (a) What are the major sources of carbon, nitrogen and phosphorous in industrial fermentation. [8] (b) What are chelating agents? Explain their functions giving example. [2+6]
4. A medium containing a vitamin is to be sterilized. Assume that the number of spores initially present is 105 /lit. The values of activation energy for the death of the organism (E0d ) and pre-Arrhenius constant for the spores are: Pre-Arrhenius constant = 1 x 1036 min-1 E0d = 65 kcal/g-mol For the inactivation of the vitamin, the values of E0d = 10 kcals/g-mol pre-Arrhenius constant = 1x 104 min-1 The initial concentration of the vitamin is 30 mg/lit. Compare the amount of active vitamin in the sterilized medium for 10 litre and 10,000 litre fermenters when broth is sterilized at 121o C when we require in both the cases that the probability of an unsuccessful fermentation be 0.001. Ignore the e?ects of heat up and cool-down periods. [16]
5. Enumerate the di?erence in prediction of yield coe?cients in anaerobic and anaerobic system with appropriate example. [16]
6. Explain the following: (a) Control sites in metabolism (b) Transfer of bioenergy via ATP. [8] [8]
7. Enumerate various environmental conditions that e?ect the growth kinetics. [16]
8. Give brief notes on structured models for growth and product formation with relevant examples. [16]
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