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Posted Date: 10 Dec 2012      Posted By:: Mahaboob Batcha    Member Level: Silver  Points: 5 (Rs. 4)

2012 University of Madras General B.Sc Biochemistry (Nov-2012) Madras University, B.Sc (Bio Chemistry), Second Year, Semester-III,BioChemical Techniques-I, SAB3A University Question paper

Course: B.Sc Biochemistry   University/board: University of Madras

Are you looking for the old question papers of Madras University B.Sc Bio-Chemistry? Here is the previous year question paper from Madras University. This is the original question paper from the B.Sc Bio-Chemistry the Third semester exam conducted by Madras University in year 2012. Feel free to download the question paper from here and use it to prepare for your upcoming exams.

University Name: Madras University
Name of the Degree: B.Sc Degree Course in Bio-Chemistry
Title of the Paper: Bio-Chemical Technologies-I
Month & Year of Examination: November - 2012
Subject Code: SAB3A
Semester: III
Year: Second Year

Time: Three hours
Maximum: 75 marks

Section A - (10 * 2 = 20 marks)
Answer any TEN questions.
All questions carry equal marks.
Each answer should not exceed 50 words.

1. What is metabolic quotient?
2. Mention the applications of manometric techniques.
3. Define pH.
4. What is buffer capacity?
5. Give these examples for buffers used in acidic medium.
6. What is an electrochemical cell?
7. Brief on alkaline error and acid error.
8. Define column chromatography.
9. How is TLC superior to paper chromatography?
10.List the factors that affect Rf value.
11.Mention the properties of an ideal matrix.
12. What is desalting?

Section B - (5 * 5 = 25 marks)
Answer any FIVE questions.
All questions carry equal marks.
Each answer should not exceed 200 words.

13. What hygiene practices should be implemented to reduce the likelihood of accident or chemical
14. Write Henderson - Hasselbalch equation and its importance.
15. Give an account on Donnan membrane equilibrium.
16. How is pH measured using a pH meter?
17. List out the different types of mobile phases used in paper chromatography.
18. Discuss the different methods of sample application in a column chromatography.
19. Explain the principle involved in ion-exchange chromatography.

Section C - (3 * 10 = 30 marks)
Answer any THREE questions.
All questions carry equal marks.
Each answer should not exceed 500 words.

20. Elaborate on mutant selection.
21. The isoionic point and isoelectric point of an aminoacid are usually identical. But in the case
of proteins it is not so. Explain.
22. What are the different types of electrodes. Brief on them and mention their uses.
23. Describe the commonly used matrix materials in column chromatography.
24. Explain the principle and operation of gas liquid chromatography.

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