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Molecular Biology

Posted Date: 23 Oct 2007 Resource Type: Articles/Knowledge Sharing Category: AdSense Revenue Sharing

Posted By: balkis Member Level: Bronze
Rating:

Points: 5

model questions
1. Unusual base present in tRNA is
(a) Adenine
(b) Pseudouracil
(c) Thymine
(d) Guanine

2. Removal of RNA primer during DNA replication in prokaryotes is catalyzed by
(a) DNA polymerase I
(b) DNA polymerase II
(c) DNA polymerase III
(d) RNA primase

3. The sigma subunit of prokaryotic RNA polymerase
(a) Must be present along with RNA polymerase for transcription to occur
(b) is part of core enzymes
(c) Specifically recognizes promoter sites
(d) is inhibited by ?-amanitin

4. Antisense RNA is complementary to
(a) antisense DNA strand
(b) sense-DNA strand
(c) dsRNA
(d) none of these

5. A chemical used to visualize DNA but with carcinogenic property is
(a) Coomassie brilliant blue
(b) Guanidium isothiocyanate
(c) SYBR-Green
(d) Ethidium bromide

6. Extracted total DNA is easily tested for intactness by
(a) Absorption spectra
(b) Agarose electrophoresis
(c) Southern hybridization
(d). PCR

7. RNA editing is common in
(a) nuclear genome
(b) chloroplast genome
(c) mitochondrial genome
(d) in all of them.

8. Transcription starts on the DNA at
(a) the middle of gene
(b) the promoter
(c) enhancer
(d) upstream of gene

9. The polymorphism within the species is easily detected by
(a) AFLP
(b) RFLP
(c) PCR
(d) RT-PCR

10. Prokaryotic genomes are composed of mostly
(a) highly repetitive DNA
(b) Moderately repetitive DNA
(c) Unique sequence DNA
(d) All of the above

11. Spliceosome assembly
(a) proceeds through a zigzag pathway
(b) excludes both snRNPs and soluble proteins
(c) needs no ATP
(d) is accompanied by multiple rearrangements of snRNPs

12. How large is a typical chloroplast genome?
(a) 1.5 kb
(b) 15 kb
(c) 150 kb
(d) 1500 kb

13. Rolling circle replication
(a) is the dominant mechanism of replication in bacteria
(b) allows for amplification of the original replicon
(c) always generates double-stranded circular copies of the original replicon
(d) is not autoregulatory in that the gene for the nicking protein resides in the replicon

14. A replicon is
(a) the segment of DNA that after replication is segregated during cell division
(b) the segment of DNA to be replicated and the enzymes and proteins needed in the process
(c) any autonomously replicated DNA sequence that is not separated in cis from origin by a terminus
(d) the product of any given replication mechanisms

15. Which base(s) in the anticodon contribute to the wobbling in codon reading?
(a) first
(b) second
(c) third
(d) first and second

16. What is the E-site of the ribosome?
(a) the site where eukaryotic mRNA is processed
(b) an exciting term made up by your instructor
(c) the site where tRNA exits in the prokaryotic ribosome
(d) the site where the endonulease EcoRI restricts the ribosome

17. The functionality of an aminoacyl-tRNA is determined by
(a) its amino acid
(b) its anticodon
(c) its invariant base regions
(d) the distance between amino acid and anticodon; the closer the better

18. The length of eukaryotic pre-mRNA is determined by
(a) a DNA stemloop sequence which produces an intrinsic terminator RNA structure in the trailer
(b) the polyadenylation site at its 3’ end at which the transcription terminates
(c) termination proteins which bind to RNA polymerase II at the termination site
(d) exonucleases which ‘trim’ all primary transcripts to final length pre-mRNAs

19. Eukaryotic genes are often interrupted which
(a) reflects the fact that eukaryotic mRNAs are polycistronic
(b) refers to the separation of coding units, the ‘exons’, by noncoding ‘introns’
(c) refers to the fact that eukaryotic DNA is linear and separated in individual chromosomes
(d) Means that the primary transcript must be processed in order to be translatable

20. The enzyme that makes DNA from RNA is
(a) RNA polymerase
(b) DNA polymerase
(c) reverse transcriptase
(d) RNAse

Responses

Author: Mala 23 Oct 2007

Member Level: Diamond Points : 2

Hi Balkis,

The questions are good. It would have been even better if the answers were too provided.

Thanks & regards,
Mala.

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