Microbial genetics

Posted Date: 24 Oct 2007 Resource Type: Articles/Knowledge Sharing Category: AdSense Revenue Sharing

Posted By: balkis Member Level: Bronze
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Microbial genetics
1. Plasmid curing is done by
a. NTG
b. Ethidium bromide
c. Acridine orange
d. TEMED

2. Proportion of mRNA in total RNA of an eukaryotic cell
a. 1-2%
b. 3-5%
c. 0.5-1%
d. 6-10%

3. Half life of mRNA in yeast
a. 10min
b. 60min
c. 20min
d. 40min

4. One gene one enzyme hypothesis was put forth by
a. Beadle and Tatum
b. Allc Jeffeys
c. Fries
d. Zinder

5. Inhibitor of pyrimidine synthesis
a. Azaserine
b. Urethane
c. UV
d. Litrous oxide

6. The receptor binding site in H.influenzae is
a. 50
b. 80
c. 12
d. 4

7. F plasmid replicate only in
a. gram +ve bacteria
b. gram –ve entric bacteria
c. gram –ve bacteria
d. all the

8. During transformation, chromosomal genes upon binding with competence factor encode
a. Competency
b. Autolysin
c. Antibody
d. None of the above

9. Transformation Mechanism is
a. Addition
b. Substitution
c. Both
d. None of here

10. Nutritional variants that require growth factors
a. autotroph
b. heterotroph
c. auxotroph
d. organotroph

11. Transformation frequency of most bacteria is
a. 10-5
b. 10-3
c. 10-6
d. 10-1

12. Vancomycin is isolated from
a. Streptomyces Ovientalis
b. Peniullium Sp
c. Bacillus polymyxa
d. Nocardia Sp

13. The rate of nucleotide mispairing after mismatch repair is
a. 5/1000cells
b. 1/2000 cells
c. 10/100 cells
d. None of these

14. In E.coli, DNA repair and replication is carried out by
a. Gryrase
b. DNA polymerase
c. Polymerase III
d. Primase

15. Chargaff’s rule is
a. A and G and T and C
b. A and T and G and C
c. G and A and T and C
d. A and U and G and C

16. Double stranded DNA runs in
a. Antiparallel
b. Parallel
c. Both
d. None of these

17. During DNA replication, RNA primer is recognized and removed by
a. RnaseH
b. Dnase
c. Primase
d. DNA pol I

18 Most of the genes for replication proteins have been found by isolating mutants defective in replication of
a) RNA
b) Protein
c) DNA
d) mRNA

19 Mutants in which the product of the gene is inactive at one temperature and active at another temperature
a) Temperature non sensitive b) Temperature Sensitive c) both d) None of these

20 Replication termination protein in E.coli
a) RTP
b) Tus
c) Oric
d) All

21 Long molecules which bind DNA on each of their ends and condense into a smaller space
a) Supercoilling
b) condensins
c) catenenes
d) none

22. The condensin of E.coli is called
a) MukB
b) SMC
c) MIN
d) MinE

23 Cell division proteins in bacteria are
a) FtsZ
b) Par A
c) Par B
d) SMC

24 Inhibitor of dihydrofolate in eubacteria
a) Trimethoprim
b) methotrexate
c) streptomycin
d) Hygromycin

25 Inhibitor of DNA precursors synthesis
a) Colchicine
b) Acirdine orange
c) Hydroxy urea
d) MMS

26 Gyrase action is inhibited by
a) ciprofloxacin
b) Thio urea
c) glucose
d) none of these

27 Smallest cell genome so far sequenced
a) Treponema Sp
b) Mycoplasma genitalium
c) Bacillus subtilis
d) E.Coli

28 Fast growing cells have ____________________DNA than slow growing cells
a) Higher
b) lower
c) both
d) none

29 Phenomena of amino acid encoding by more than one codon.
a) Degeneracy
b) Rederndamey
c) complementation
d) hobbling

30 Set of genes regulated by the same regulatory gene product
a) regulan
b) codon
d) introns
d) exons
31 RNA synthesis is inhibited by
a) Rifampin
b) Thiourea
c) lysozyme
d) penicillin

32 Antibiotic which bind to 23S RNA of gram +ve bacteria and block translation
a) Novotriocin
b) Tetracyclin
c) Thiostrepton
d) Streptomycin

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