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GATE syllabi XE - Engineering Sciences BIOTECHNOLOGY
Posted Date: 06 Dec 2007 Resource Type: Articles/Knowledge Sharing Category: General
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Posted By: Bala Member Level: Diamond
Rating:  Points: 1
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SECTION L. BIOTECHNOLOGY
Advanced techniques in gene expression and analysis: PCR and RT-PCR, microarray technology, DNA fingerprinting and recombinant DNA technology; prokaryotic and eukaryotic expression systems; Vectors: plasmids, phages, cosmids and BAC.
Architecture of plant genome; plant tissue culture techniques; methods of gene transfer into plant cells and development of transgenic plants; manipulation of phenotypic traits in plants; plant cell fermentations and production of secondary metabolites using suspension/immobilized cell culture; expression of animal protein in plants; genetically modified crops.
Animal cell metabolism and regulation; cell cycle; primary cell culture; nutritional requirements for animal cell culture; techniques for mass culture of animal cell lines; application of animal cell culture for production of vaccines, growth hormones; interferons, cytokines and therapeutic proteins; hybridoma technology and gene knockout; stem cells and its application in organ synthesis; gene therapy; transgenic animals and molecular pharming.
Industrial bioprocesses: microbial production of organic acids, amino acids, proteins, polysaccharides, lipids, polyhydroxyalkanoates, antibiotics and pharmaceuticals; methods and applications of immobilization of cells and enzymes; kinetics of soluble and immobilized enzymes; biosensors; biofuels; biopesticides; environmental bioremediation.
Microbial growth kinetics; batch, fed-batch and continuous culture of microbial cells; media for industrial fermentations; sterilization of air and media, design and operation of stirred tank, airlift, plug flow, packed bed, fluidized bed, membrane and hollow fibre reactors; aeration and agitation in aerobic fermentations; bioprocess calculations based on material and energy balance; Down stream processing in industrial biotechnology: filtration, precipitation, centrifugation, cell disintegration, solvent extraction, and chromatographic separations, membrane filtration, aqueous two phase separation.
Bioinformatics: genomics; proteomics and computational biology.
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