Microbial Physiology Metabolism - Syllabus
Duration of Examination: 3 Hours
Max. Marks: 100
Major Biomolecules: Carbohydrates - Classification, chemistry, properties and function - mono, di, oligo and polysaccharides. Conjugated polysaccharides- glycoproteins, muriens and lipopolysaccharides. Lipids - classification, chemistry, properties and function - free fatty acids, triglycerides, phospholipids, glycolipids & waxes. Conjugated lipids - lipoproteins. Major steroids of biological importance - prostaglandins.
Amino acids and proteins - classification, structure and function. Essential amino acids & amphoteric nature of amino acids. Reactions and functions of carboxy and amino groups and side chains. Peptide structure Ramachandran's plot. Methods for isolation and characterization of proteins. Structural levels of proteins - Primary, secondary, tertiary and quaternary, denaturation of proteins - Hydrolysis of proteins.
Protein sequencing using various methods. Nucleic acids - structure, function and their properties. Structural polymorphism of DNA, RNA. Structural characteristics of RNA.
UNIT - III
Nutritional types - autotrophic bacteria, chemosynthetic and photo synthetic micro organisms, Heterotrophic bacteria - saprophytes, parasites and mixotrophs. Respiration in bacteria - aerobic and anaerobic types of Respiration, obligate aerobes, facultative anaerobes and obligate anaerobes. Toxic effect of oxygen on anaerobes. Bioluminescence in microorganisms. Carbohydrate metabolism in microbes - synthesis of Carbohydrate in photosynthetic, chemosynthetic and heterotrophic microbes. Fermentation of carbohydrates by microorganisms - Embden-Meyerhof-Parnas pathway, Entner-Doudoroff (ED) pathway, C2-C4 split Pathway Kreb's cycle, glyoxylate cycle, hexose monophosphate shunt (HMP), gluconeogenesis, synthesis of peptidoglcans and glycoproteins.
The Electron Transport Chain (ETS), mechanism of oxidative phosphorylation. Inhibitors of ETS and oxidative phosporylation. Lipid metabolism in bacteria. Metabolism of triglycerides, biosynthesis & ß -oxidation of fatty acids. Mineral metabolism - phosphorus, potassium, calcium and Trace elements – molybdenum, zinc, manganese, cobalt and copper. Influence of minerals on the production of toxins. Role of trace on microbial enzymes.
UNIT – V
Metabolism of amino acids - Biosynthesis and catabolism of histidine, aromatic and branched chain amino acids by microbes. Biosynthesis and catabolism of purine and pyrimidine nucleotides, salvage pathways. Sources, Chemistry and biochemical functions of water soluble vitamins. Chemistry of Porphyrins - Heme, Cytochromes, Chlorophylls, Bacteriochlorophylls algal pigment.
1 . Text book of Biochemistry by West E.S. Tood. Oxford & IBM Publishing Co. Pvt. Ltd
2. Biochemistry by Donald Voet, Judith G Voet, John Wiley & Sons, Inc
3. Biochemistry by Lubert Stryer.
4. Principles of Biochemistry by Lehninger, Nelson & Cox, CBS Publishing & Distributors.
5. Biochemistry by I Harper McGraw Hill
6. Principles of Biochemistry by Cohn & Stamph, John Wilcy & sons Inc
7. Biochemistry of Nucleic acids by Davidson.
8. Biological chemistry by I-, Mullar & Cords
9. Biochemistry by White, Handler & Smith.
10. Microbial Physiology by Moat
11. Bacterial Metaboism by Dwelley
No responses found. Be the first to respond...