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ADIKAVI NANNAYA UNIVERSITY-M.Sc.Zoology– III- QUANTITATIVE BIOLOGY,TOOLS AND TECHNIQUES
Posted Date: 31 Aug 2008 Resource Type: Articles/Knowledge Sharing Category: Syllabus
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Posted By: Nitin Bajaj - Editor Member Level: Diamond
Rating:  Points: 2
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ADIKAVI NANNAYA UNIVERSITY
RAJAHMUNDRY
M.Sc(Zoology) Syllabus
Z 10 – QUANTITATIVE BIOLOGY,TOOLS AND TECHNIQUES
Unit 1
1.0 Biostatics:
Introduction and scope of biostatics in biology,concept of sampling ,frequency
Distribution ,sampling errors ,sample size,probability
Graphic presentation of data,measurement of central tendency, mean mode and
Median ,standard deviation and standard error
Distribution:binomial,poisson and norma
Unit 2:
2.0 Test of significances, paired and unpaired t-test,chi-square analysis
2.1. Analysis of bvariance
2.3. Correction and regression
Unit 3:
3.0 Biological Assays
3.1 Definition and criteria of reliability
3.2 Chemical assays
3.3 Biological assays- in vivo and in vitro assys
3.1 Principles and uses of analtytical instruments – balances,pH meter,colorimeter,
Spectrophotometer,ultracentrifuge,densitometer scanner,spectrophotometer,
Radioactivity counter, ESR and NMR spectrometers.
Unit 4:
4.0 Microscopy-Principles of light tsmission,electron,phasecontrast,fluorescene
scanning electron microscopes,M icrophotography image analysers.
5.0 Cell culture techniques
5.1 Laboratory facilities
5.2 Treatment of substrate surfaces
5.3 Feeder layers
5.4 Culture media
Unit 5:
6.0 Separation techniques in biology
6.1 Molecular separation by chromatography,electrophoresis,precipitation etc.
6.2 Cell separation by density gradient centrifugation ,gravity centrifugation,affinity,
adsorption,and anchorage basestechniques etc.
6.3 Computer aided techniques for data presentation,data analyses, statistical techniques
Practical:
1. Spectrophotometer-estimation of biomolecules
2. Centrifugation-Demonstration and working
3. Separation Techniques- Paper chromatography
4. Electrophoresis-Demonstration andusage
5. Demonstration and working of Atomic Absorption Spectrophotometer
6. High Pressure Liquid Chromatography
7. ELISA Reader
8. Liquid Scintillation counter
Suggested books.
1. Animal cell culture- A Practical approach,Ed.John R.W.Masters, IRI Press
2. Introduction to Instrumental Analysis Robert Braun Mc Graw Hill International
editions
3. A Biologist 3Guide to Principles and Techniques of Practical Biochemistry
K.Wilson and K.H.Geoulding,ELBS Edn.
4. Batschelet.E. Introduction to mathematics for life scientists.Springer Verlag
Berlin
5. Jorgenson S.E.Fundamentals of Ecological modeling.Elsesvier,New York.
6. SwaritzmanG.L and S.P.O.Kaluzny.Ecologycal simulation .primer Mc Millan
New York.
7. Lendren D.Modelling in Behaviour Ecology .Chapman&Hall,London.
8. Sokaii,R.R. and F.J.Rohlf.Biometry .Freeman, San Franscisco.
9. Murray J.D.Mathematical Biology.Springer Verlag,Berlin.
10. Pielou,E.C.The interpretation of Ecological data.A Primer on classification
and ordination
For more details, visit http://www.nannayauniversity.info/courses.html
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