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316281: Biochemistry II
Teaching Scheme: Exam Scheme:
Theory: 4 hr/week Paper: 100 Marks
Practical: 2 hr/week Practical: 50 Marks
UNIT 1 [9 Hrs]
Enzymes
Structural aspects, isozymes and multienzymes complexes, cofactors, activation, catalytic
mechanisms with reference to specific examples. Enzyme kinetics; Michalies-Menten
Equation, inhibition, competitive/non-competitive/un-competitive, allostersric and feedback
inhibition. Enzymatic activity of molecules other than proteins.
UNIT 2 [9 Hrs]
Coezymes
Coenzyme I -Coenzyme A, Thiamine diphosphate, pyridine nucleotides, flavins and lipoic
acid Coenzyme II Biotin and pyridoxal phosphate
UNIT 3 [9 Hrs]
Receptors and ligand binding, agonist, antagonist with case examples. NF-kappa b, PPAR,
cannabinoid receptors, GPCR
UNIT 4 [9 Hrs]
Biochemical structures
Proteins, nucleic acids, lipids: secondary structure, tertiary structure, Ramchandran Plot,
structure-function correlations, anatomy of biological macromolecules.
UNIT 5 [9 Hrs]
Basic physiology, homeostasis. Hormones, endocrine and exocrine systems. P-450 drug
metabolizing enzymes and metabolism of drugs. Lysosomes and degradation of proteins.
Muscle contraction, nerve conduction, action potential, signaling pathways, signal
transduction, Nerve conduction, compartmentalization, membrane channels and pumps (Na/k
pumps, other such examples)
UNIT 6 [9 Hrs]
Clinical Biochemistry, application of biochemistry in monitoring systemic diseases. vitamins.
assimilation, macronutrients and micronutrients, vitamins and trace elements, chemistry and
3
metabolism. Nutritional Biochemistry, Its application in food technology (yogurt,cheese,wine
etc.)
Practical:
1. Water: analysis and methods of preparation
Conductivity
2. Isolation of complex polysaccharides using solvent extraction
3. Isolation of proteins by Salting in and out
4. Separation of proteins by SDS PAGE
5. To carry out purification of enzyme from liver or sprouts or microbial source such as
Proteases, or Amylases, or lipases etc and calculate specific activity
6. Quantitative assays for enzymes using enzyme substrate reactions
7. To study enzyme kinetics and calculate Km and Vmax
8. To study effect of enzyme inhibitors and calculate Ki
Text Books
1. Lubert stryen Biochemistry, Freeman WH & Company, New York,
2. Conn and Stumph, Outlines of Biochemistry
Reference Books
1. JH Weil, General Biochemistry, New Ages International (P) Ltd. 1997.
2. David T. Plummer, An Introduction to practical biochemistry, Tata McGraw
Publishing Company Ltd.
3. I.A.L. Lehninger, DL Netson, MM Cox Principles of Biochemistry, CBS Publishers
and Distributors.
For more details, visit http://www.unipune.ernet.in/stud_info/Syllabi/Syllabus_2008.html
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