Osmania University - M.Sc Botany- 4 -(T)(P) Paper-1.Molecular Genetics, Biostatistics, Eco-diversity

Practicals
Section - A

1. Problems in Restriction mapping of plasmids.
2. Problems in Biostatistics:
a.Graphic representation of data: Histogram.
b.Mean, Variance, Standard Deviation and Standard Error.
c.Chi-square and Student’s “t” test.
d.Concept of Probability: Addition and multiplication rule.
3. Isolation of DNA.
4. Demonstration of plant tissue culture methods.
5. Maintenance of Practical Record.

Section - B

The practical course comprises (a) Field survey and (b) Scientific visits.

a) Field survey:

1. Survey of the campus to be undertaken (by selecting one avenue/road by individual students) to locate the planted trees on a graph paper and collect details of identification, size, canopy shape, blossoming and fruiting period and their status (healthy, diseased, infested, mutilated, misused or dying). The individual reports may be combined to prepare a larger map of the area to be available for future batches of students / civic authorities.
2. Prepare and record a short list of 10 most important sources of firewood and timber in your locality. Give their local names, scientific names and families to which they belong. Mention their properties.

b). Scientific visits:

The students should be taken to one of the following:
1. A national park / sanctuary.
2. A wet land
3. A mangrove.
4. National Bureau of Plant Genetic Resources, New Delhi or its field stations.
5. Head quarters of Botanical Survey of India or one of its Regional circles.
6. A CSIR laboratory doing research on plants and their utilization.
7. An ICAR Research Institute or a field station dealing with major crops.
8. A recognized Botanical garden or museum (like The Forest Research Institute, Dehra Dun, National Botanical Research Institute, Lucknow, Tropical Botanical Garden and research Institute, Trivandrum), which has rich collection of plant products.

Note: The students must prepare and submit a brief illustrated record of the field survey and scientific visits. After evaluation, the grades awarded to the students by the teachers should be added to the final assessment of the practical examination.

List of books recommended
Section – A

1. Watson, Gilman, Wittkowsky and Zoller. 1992. Recombinant DNA.
2. Rastogi, Sharma and Tandon 1994. Concepts in Molecular Biology.
3. Snustad, W and Simmons Wiley.1991. Principles of Genetics.
4. Shaw, G. M. 1988. Plant Molecular Biology. A practical approach. Ed.
5. Davis, L, Kuehl and Battey. 1994.Basic methods in Molecular Biology. Ed.
6. Twyman. 1998. Advanced Molecular Biology.
7. Turner, Mclennon, Bates and White. 1999. Instant notes in Molecular Biology.
8. Primrose. 1999. Molecular Biotechnology.
9. Hughes, M. A. 1992. Plant Molecular Genetics.
10. Prathibha Devi. Principles & Methods in Plant Molecular Biology,Genetics & Biochemistry,,Agrobios.
11. Purohit. S. S. 1999. Agricultural Biotechnology.
12. Stansfield. 1996. III Ed. Theory & Problems in Genetics. Schaum’s Series. McGraw & Hill.
13. Khan, I. A. and A. Khanum. 1994Fundamentals of Biostatistics
14. B. N. Mishra and K. K. Mishra. Naya Prakash. 1983. Introductory practical Biostatistics.
15. Jain, v. k. Computers for beginners. Pustak Mahal.
16. Vikas Gupta, 2000. Rapidex computer course. Rapidex series.
17. Bennet Falk. 2000. The Internet road map. BPB Publications.
18. BPB Publications 2000. The ABCs of Internet..
19. Cynthia Gibas. O’Reilly & Assoc.2000. Developing Bioinformatics Computer skills.
20. Balasubramainan. Ed. Concepts in Biotechnology.Universities Press.1996.
21. Deepak Bharihoke.2000. Fundamentals of Information technology.
22. Crumlish. 1999. The Internet.Ed.
23. Gralla. 2000. How the Internet works.
24. White.2000. How computer works.

Section B:

1. Anonymous 1997. National Gene Bank: Indian Heritage on Plant Genetic Resources (Booklet). National Bureau of Plant Genetic Resources, New Delhi.
2. Arora, R.K. and Nayar, E.R. 1984 Wild relatives of crop plants in India. NBPGR Science Monograph No. 7.
3. Baker, H.G. 1978. Plants and Civilization (3rd Ed. C.A. Wads worth, Belmont.
4. Bole, P.V. and Vaghan, Y. 1986. Field Guide to common Indian trees. Oxford Univ. Press, Mumbai.
Chandel, K.P.S. Shukla, G. and Sharma, N. 1996. Biodiversity in Medicinal and Aromatic plants in India. Conservation and Utilization. National Bureau of Plant Genetic Resources, New Delhi.

Osmania University - M.Sc Botany- 4 -(T)(P) Paper-1.Molecular Genetics, Biostatistics, Eco-diversity

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