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Osmania University - M.Sc BIOTECHNOLOGY - 3 - BTU 303 Immunology
BTU 303 Immunology
Credit I Basics of Immunology
1.1 Immunity – Types of Immunity, Innate and Acquired Immunity
1.2 Cells of the Immune System – B & T Lymphocytes; T-cell sub-sets; The Antigen Presenting Cells
1.3 Organs of the System : Primary lymphoid organs (Bone marrow and Thymus); Secondary lymphoid organs (lymph nodes, spleen and mucosal-associated lymphoid tissue)
1.4 Antigens - Immunogenecity versus Antigenicity, Factors that influence immunogenecity, Epitopes - Properties of B-cell epitopes and T-cell epitopes, Haptens and the study of Antigenicity
Credit II Immunoglobulins : Structure and Functions
2.1 Basic structure of Immunoglobulins - The role of multiple myeloma in understanding Ig structure
2.2 Fine structure of Immunoglobulins - Immunoglobulin domains-variable region and constant region domains
2.3 Immunoglobulin classes - IgG, IgM, IgA, IgD and IgE; functions of Ig classes
2.4 Organization and expression of Immunoglobulin Light and Heavy chain genes
2.5 Monoclonal Antibodies - Formation and selection of hybrid cells; Production of Monoclonal Antibodies and their clinical uses
Credit III Major Histocompatibility Complex (MCH)
3.1 General organization and inheritance of MHC; MHC Haplotypes
3.2 The structure of MHC class-I and class-II molecules; organization of MHC class I and class II genes, peptide binding of MHC molecules
3.3 Polymorphism of MHC class I and class II molecules; the role of HLA typing in organ transplantation
3.4 Cellular distribution of MHC molecules; MHC molecules and immune responsiveness
Credit IV The Humoral and Cell-mediated Immune Responses
4.1 Antigen processing by antigen-presenting cells
4.2 The structure and functions of T-cell receptors ( TCR ); the TCR-peptide-MHC tri-molecular complexes
4.3 B-cell activation and proliferation by Thymus independent and Thymus dependant antigens; invivo sites for induction of humoral response; B-cell differentiation, class-switching and generation of plasma cells and memory cells
4.4 Cell-mediated immune response: General properties of effector T-cells; Direct cytotoxic response; experimental assessment of cell-mediated cytotoxicity; Delayed type of hypertensivity (DTH) and cytokines involved in DTH
4.5 Auto-immunity and auto-immune diseases-Insulin Dependant Diabetes; Rheumatoid Arthritis and Auto-immune Thyroid disease
1. Micro-hemagglutination Test
2. Single Radial Immunodiffusion
3. Enzyme-Linked Immuno-sorbent Assay
4. Cell-viability Test by Trypan Blue
5. Differentiation of titers of Rheumatoid Arthritis
6. Principle and procedure for Enumeration of specific blood types by FACS ( Florescent Activated Cell Sorter )
7. Demonstration of Western Blot by Enzyme-conjugated antibody
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