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JNTU 2007-2008 II Year I Sem. B. Tech. BME -Applied Chemistry and Biochemistry
Posted Date: 14 Dec 2007 Resource Type: Articles/Knowledge Sharing Category: Syllabus
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Posted By: Sri Member Level: Gold Rating: Points: 1
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JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD II Year B.Tech. BME – I Semester T P C 4+1* 0 4 APPLIED CHEMISTRY AND BIOCHEMISTRY UNIT-I Electro Chemistry: Conductance, Specific Equivalent and Molecular Conductance -Cell constant -Measurement of Equivalent Conductance –Galvanic Cell Standard Electrode Potentials -Calculation of E. M. F of a cell –Determination of pH by E. M. F methods.
UNIT-II Polymers: applications of Polyethylene, Teflon, PVC, Bakelite, Rubber -Natural Rubber.
UNIT-III Lubricants and water Technology: Definition -Theory of Lubrication, properties of Lubrication. Nature of Impurities present in water, Hardness of water and its Determination, Different methods of Treatment of water- Lime soda, Zeolite, Ion exchange process- Analysis of water.
UNIT-IV Biochemistry of Living Cell, Sub-cellular. Fractionation using the Differential Centrifugation Method. Functions of each Organelle, Transport of Substances across Biological Membrane Function. Redox potential, Oxidative phosphorylation. UNIT-V Chemical nature of Enzymes - study of the Properties of Enzymes and kinetics by Spectrophotometer. Diagnostic and therapeutic uses of Enzymes.
UNIT-VI Carbohydrate, Protein & Lipid metabolism (Briefly), Nucleic Acid chemistry. Protein synthesis. Transcription and Translation, Replication, Polymerase Chain Reaction(PCR) Immunological Techniques or Immunoassay – Radio Immuno Assay (RIA), Enzyme- Linked Immunosorbent Assay (ELISA), Chemiluminiscence.
UNIT-VII Blood Chemistry: Chemical Composition of Blood, Separation of Serum Proteins and lipoproteins by Electrophoresis and Ultracentrifugation Acid Base Balance and Biochemical Measurements of Acid-Base and Electrolyte status of the patients, Urine Analysis.
UNIT-VIII General methods of biochemical analysis carried out in the estimation of blood constituents, such as glucose etc. Principles and different methods of chromatography – fluorometry, flame photometry, Automation and Biochemical Analysis. Applications of isotopes in biochemistry.
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