UNIT I OVERVIEW OF FERMENTATION PROCESSES 12
Fermentation process – Medium requirements – Medium formulation of optimal growth and product
formation – Simple and complex media – Design of various commercial media for industrial
fermentations – Microbial growth – Applications of fermentation – Microbial biomass – Microbial
metabolites – Microbial enzymes – Transformation process – Recombinant products – Main
parameters in fermentation processes – Mode of operation of fermentation processes – Basic
configuration of fermenter and axillaries.
UNIT II KINETICS OF IMMOBILIZED ENZYMES AND STERILIZATION 12
Methods of immobilization – Kinetics of immobilized enzymes – Thermal death kinetics of
microorganisms – Batch and continuous heat sterilization of liquid media – Filter sterilization of
liquid media – Air sterilization and design of sterilization equipment.
UNIT III METABOLIC STICHIOMETRY AND ENERGITICS 12
Stoichiometry of cell growth and product formation – Elemental balances – Degrees of reduction of
Substrate and Biomass – Available Electron Balances – Yield coefficients of Biomass and Product
formation – Maintenance coefficients energetic analysis of microbial growth and product formation –
Oxygen consumption and heat evolution in aerobic cultures – Thermodynamic efficiency of growth.
UNIT IV KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATION 12
Modes of operation – Batch, Fed Batch and Continuous Cultivation – Simple unstructured kinetic
models for microbial growth – Monad model – Growth of filamentous organisms – Product formation
kinetics – Leudeking-piret models, substrate and product inhibition on cell growth and product
UNIT V OPTIMIZATION METHODS 12
Overview of response surface methodology – Factorial design – Central composite design – Three
level design – Plackett-Burman design and Taguchi design of experiments.
L: 60 Total: 60
1. Stanbury P. F., Hall S. J. and Whitaker A., “Principles of Fermentation Technology”, Science
& Technology Books, 1999.
2. Blanch H. W. and Clark D. S., “Biochemical Engineering”, CRC Press, 1997.
1. Anderson M.J. and Whitcomb P.J., “RSM Simplified: Optimizing Processes Using Response
Surface Methods for Design of Experiments”, Productivity Press, 2005.
2. Jack P.C. Kleijnen, “Design and Analysis of Simulation Experiments”, Springer, 2008.
3. Roy R.K., “Design of Experiments Using the Taguchi Approach: 16 Steps to Product and
Process Improvement”, Wiley-IEEE, 2001.
4. Shuler M.L. and Kargi F., “Bioprocess Engineering: Basic Concepts”, Second Edition,
Prentice Hall, 2001.
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