BIOCONJUGATE TECHNOLOGY

UNIT I FUNCTIONAL TARGETS 9
Modification of amino acids, Peptides and proteins – Modification of sugars, polysaccharides
and glycoconjugates – Modification of nucleic acids and oligonucleotides.
UNIT II CHEMISTRY OF ACIVE GROUPS 9
Amine reactive chemical reactive chemical – Thiol reactive chemical reactions – Carboxylate
reactive chemical reactions – Hydroxyl reactive chemical reactions – Aldehyde and ketone
reactive chemical reaction – Photoreactivc chemical reactions.
UNIT III BIOCONJUGATE REAGENTS 9
Zero length cross linkers – Homobifuntional cross linkers – Heterobifunctional cross linkers
– Trifunctionaal cross linkers – Cleavable reagent systems –Tags and probes.
UNIT IV ENZYME AND NUCLEIC ACID MODIFICATION AND
CONJUGATION 9
Properties of common enzymes – Activated enzymes for conjugation – biotinylated enzymes
–chemical modification of nucleic acids – Biotin labeling of DNA enzyme conjugation to
DNA – Fluorescent of DNA.
UNIT V BIOCONJUGATE APPLICATIONS 9
Preparation of Hapten – Carrier immunogen conjugates – Antibody modification land
conjugation – Immunotoxin conjugation techniques – Liposome conjugated and derivatives –
Colloidal – Gold –Labeled proteins – Modification with synthetic polymers.
Total: 45
TEXT BOOK
1. Hermanson, G.T., “Bioconjugate Techniques", 2nd Edition, Academic Press,
2008.
REFERENCES
1. Niemeyer, C.M, “Bioconjugation Protocols: Strategies and Methods " Springer, 2004.
2. Aslam, M. and Alastair, D., “Bioconjugation: Protein Coupling Techniques for the
Biomedical Sciences" Macmillan, 1998.